KMID : 0648620080130020083
|
|
Korean Journal of Nosocomial Infection Control 2008 Volume.13 No. 2 p.83 ~ p.89
|
|
Rapid Screening of Vancomycin Resistant Enterococci with Chromogenic Agar (ChromID VRE)
|
|
Choi Tae-Yeal
Kang Jung-Oak Pai Hyun-Joo Pai Hyun-Joo
|
|
Abstract
|
|
|
Background: Rapid screening of vancomycin-resistant enterococci (VRE) is very important for controlling and preventing the spread of VRE in hospitals. We compared the performance characteristics of a chromogenic agar (ChromID VRE, bioMerieux, France: CA) to that of Enterococcosel agar (supplemented with 6 microgram/mL of vancomycin :EA) for direct detection of VRE from stool swabs.
Methods: Total 125 rectal swabs were collected from 57 patients in the intensive care units of an 850-bed university hospital over a period of 3 months. The samples were inoculated on EA, CA and into broth enrichment containing 6 microgram/mL of vancomycin (BE). BE was subcultured on CA after overnight incubation.
Results: Eighty two samples from 22 patients were positive for VRE by BE. At 24 h, the sensitivity/specificity of EA and CA were 89%/100% and 72%/100%, respectively. At 48 h, the sensitivity/specificity of EA and CA were 94%/89% and 89%/100%, respectively.
Conclusion: CA provides equivalent sensitivity comparable to EA for the recovery of VRE at 48 h incubation, and has additional advantage of being able to differentiate between vancomycine resistant E. faecium and E. faecalis.
|
|
KEYWORD
|
|
Vancomycin resistant enterococci (VRE), Enterococcosel, ChromID VRE
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|